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sample glp1r negative  (Alomone Labs)


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    Alomone Labs sample glp1r negative
    Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample <t>GLP1R</t> negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001
    Sample Glp1r Negative, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sample glp1r negative/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    sample glp1r negative - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons"

    Article Title: Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons

    Journal: Nature Communications

    doi: 10.1038/s41467-019-09045-9

    Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample GLP1R negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001
    Figure Legend Snippet: Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample GLP1R negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001

    Techniques Used: Expressing, In Vitro, Blocking Assay, Imaging, Transfection, Derivative Assay



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    sample glp1r negative  (Alomone Labs)
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    Alomone Labs sample glp1r negative
    Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample <t>GLP1R</t> negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001
    Sample Glp1r Negative, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sample glp1r negative/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    sample glp1r negative - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample GLP1R negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001

    Journal: Nature Communications

    Article Title: Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons

    doi: 10.1038/s41467-019-09045-9

    Figure Lengend Snippet: Expression analysis and pharmacological characterization of ATP receptors in nodose (ND) ganglion neurons. a P2rx subunit expression levels (2 −ΔCt values) of ND neurons from intact ganglia (black circles), acutely dissociated neurons (black squares), and after 3 days in vitro cultures (black triangles). Samples for each type of preparation were prepared from ND ganglia pooled from 2 to 3 mice, repeated three independent times. Individual data points represent independent preparations and lines represent mean ± SEM ( n = 3). b Heat map of P2rx subunit expression from individually picked ND neurons. Each column represents a single ND neuron. Range indicator for heat map on left. Sample GLP1R negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R. Scale bars represent 20 µm. e Scatterplot of % block of exogenous ATP (100 µM) application by 100 µM PPADs (grey filled circles, n = 27), 100 µM suramin (grey filled squares, n = 22), 30 µM AF-353 (grey filled triangles, n = 48) or 1 µM Ro51 (half grey filled triangles, n = 72) as measured by Fura2 Ca 2+ imaging. Individual data points represent individual neurons and lines represent median ± interquartile range. f Intracellular Ca 2+ levels, as the measured Fura2 signal (340/380 nm ratio), of an mCherry-positive Gq-DREADD transfected GLUTag cell (top trace) and GLP1R-positive ND neuron (lower trace) identified using a GLP1R-Cre/GCaMP3 reporter mouse. Drugs were applied as indicated above the traces. g Amplitude of Fura-2 ratio changes of ND neurons in response to CNO (10 µM) with and without Ro51 (1 µM) pre-treatment. Values are expressed relative to the max response elicited by ATP (100 µM) in each cell. Individual data points represent each ND neuron ( n = 14). Dark purple symbols represent identified GLP1R-positive ND neurons. Statistical analysis performed using a paired t -test, ** p < 0.01. h Percentage block of CNO responses in ND neurons by 1 µM Ro51, derived from the data shown in g . Individual data points represent each ND neuron and lines represent mean ± SEM ( n = 14). Statistical analysis performed using one-sample t -test, *** p < 0.001

    Article Snippet: Sample GLP1R negative ( c ) and GLP1R-positive ( d ) NeuroD1-EYFP neuron immunostained for P2X 3 (Alomone P2X 3 antibody APR-016 in c , Neuromics P2X 3 antibody GP10108 in d ) and GLP1R.

    Techniques: Expressing, In Vitro, Blocking Assay, Imaging, Transfection, Derivative Assay